A multitude of groups around the world generously contributed data to further characterise the performance of KOLF2.1J, and the project was made possible by the tireless efforts of Caroline Pantazis, Andrian Yang, Erika Lara, Justin McDonough, Cornelis Blauwendraat, Liong Peng, John Marioni, and many others. The substantial team effort was initiated by Michael Ward and Mark Cookson at the NIH who co-lead the iPSC neurodegenerative disease ( INDI) consortium and Bill Skarnes at JAX whose team has developed highly efficient protocols for gene editing. The cell line and its derivatives are now available with minimal restrictions and cost to the research community via a user-friendly website from the Jackson Laboratories (JAX).
Together we found one cell line, KOLF2.1J, that performs well across the board after we deeply analyzed its genetic background and stability, and its ability to be efficiently gene edited by CRISPR/Cas9 and differentiated into many different functional cell types. Continue to develop approach as better technology emerges (e.g.Over the past few years, we have been working on an internationally collaborative project to identify a human induced pluripotent stem cell (iPSC) line that performs well across the board to underpin large-scale collaborative studies.On track to have first 7 Cre lines made by 9/2010 and characterized by 12/2010.Future enhancer cloning will be very simple and efficient.2 enhancer elements are ready for targeting into JM8A3 ES cells (this month).2 BAC Cres (Tbx22 and p63) are cloned, Krt6 is nearly done (unique challenges).All basic cassettes (CreIRESmCherry, ROSA targeting vector, etc) are complete.Initial plan is to make four BAC transgenics (Krt6-Cre, Tbx22-CreERT2, dNp63-CreERT2, Lhx7/8-Cre) and three enhancer (Visel project) CreERT2 lines (R26 knock-in).Goal: To generate 15 Cre driver strains for orofacialclefting research.new ENU induced mutant strain (on FVB.A) įaceBaseCre Driver Project:Current progress and future plans.
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